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Rb antibody [2B5]

Anti-Rb antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634627
Anti-Rb antibody [2B5] used in Western Blot (WB). GTX634627

Cat. No. GTX634627

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B5

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human Rb.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888467

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rb antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634627

GTX634627 ICC/IF Image

Rb antibody [2B5] detects Rb protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rb stained by Rb antibody [2B5] (GTX634627) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-Rb antibody [2B5] used in Western Blot (WB). GTX634627

GTX634627 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody [2B5] (GTX634627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations